: Utilizing The program for your qPCR thermocycler, identify at which cycle the fluorescence intensity crosses a person‐specified threshold. Applying beneficial and detrimental controls as benchmarks, pick a threshold that Evidently distinguishes amplification from non‐amplification. If a solution of proteinase K (PK) is used for sample assortment (Essential Protocol 2), https://www.pinterest.com/pin/993958580252567205/